Download Der Stickstoffumsatz / Nitrogen Metabolism by W. Ruhland (Editor), E.Ashby (Editor), J. Bonner (Editor), PDF

By W. Ruhland (Editor), E.Ashby (Editor), J. Bonner (Editor), M. Geiger-Huber (Editor), W.O. James (Editor), A. Lang (Editor), D. Müller (Editor), M. G. Stålfelt (Editor)

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VAN bERSON et al. 1940, p. ) Perhaps it was the genius of BEIJERINCK that enabled him to draw the correct conclusion from this type of experiment (although note that even he believed for a time that the growth of the azotobacter in the selective culture arose from a symbiosis with other nitrogen-fixing species), but, in general, ocular assay is too frail a reed for the majority of us to lean heavily upon (WILSON 1940, p. 98, WILSON 1952). The biochemically-minded investigator who relies too confidently on some modification of the Kjeldahl method may encounter other pitfalls.

When oximes are in solution, they dissociate to form the free base and the keto compound. The amount of dissociation depends on the particular oxime and can be reduced partially by adding an excess of the 23 Minerals. keto component. After dissociation, the free base can decompose to yield NHa and other products; thus the oxime acts essentially as a reservoir for a low level of ammonia. In long-time experiments such dissociation could give rise to an apparent utilization of the oxime. The slow and incomplete utilization of oxime-N observed by several investigators (ROSENBLUM and WILSON 1951, SEGAL and WILSON 1949, WOOD et al.

III. Enzymatic mechanism. 1. Introduction. BURK (1934) and his collaborators initiated the investigations that furnished the major knowledge of the enzyme system responsible for N 2-fixation in the Azotobacter. The time was propitious for such an attack. The biochemical methods available then had about exhausted their usefulness so that there was little expectation that much additional information could be obtained through application of these methods. The major contributions of BURK and his associates were The pN2 function.

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